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Easyroti
Easyroti

Easyroti

Easyroti is liquid marine phytoplankton specifically formulated for rotifer culture.

Net content: 250, 1500 y 3000 ml.

Two microalgae species Nannochloropsis (80 %) + Tetraselmis (20 %) are used for rotifer culture.

The expiration date does not change even if it has started to be used. The reason for the expiration date not changing is because the bag compresses as the gel comes out. This prevents air from entering the interior of the bag and thus prevents the oxidation of the phytoplankton.

Preservation. Store Easyroti in a dark, dry and cool place. The storage temperature should not exceed 25°C. In summer, if temperatures are higher, it is recommended to store it in an air-conditioned room.

Nannochloropsis (80 %) + Tetraselmis (20 %).

Cellular concentration: 5 x 109 cells/ ml

It is advisable to distribute the dosage of Easyroti throughout the day.

For a correct dispersion, it is necessary that Easyroti is added to the culture tank, previously diluted in water from the same culture.

Easyroti can be automatically added using a dosing pump as long as the correct dispersion of the product is guaranteed.

To maintain a rotifer culture, follow the following directions:

  • Necessary material: 3 cylindrical recipients of 2 L with soft aeration, using a hollow rod. Initial rotifer inoculum.
  • Necessary feed: Easyroti: Nannochloropsis (80 %) + Tetraselmis (20 %).
  • Culture cycle from strain: 5 days

We will refer to “sea water” as water prepared using artificial salts for marine aquarium at a density of 1019-1022.

Water temperature should be maintained in the range of 20-28°C, being optimal at 24°C.

A 50-micron mesh will be used to filter rotifer.

It consists of a parallel culture system in which from day 6 food is obtained continuously for the aquarium. One container for “Strain” and another for “Production”.

The dosage information shown below are under ideal conditions, assuming the ideal rotifer density. Sometimes the amount of microalgae added may not meet rotifer’s demand. In the case of microalgae depletion (the water becomes transparent and yellowish), it will indicate that the dosage has not been sufficient. When rotifers remain without feed for more than 4 hours, the number of eggs decreases, and therefore the population growth rate decreases as well. It is essential that rotifer always has feed available.

Rotifers are very sensitive to changes in the physical and chemical parameters of water. If water quality worsens rapidly, the rotifer population will be affected. If excessive dirt is observed in the recipient, filter the rotifer and inoculate it into a clear container and add the necessary algae.

With experience, the intensity of the green color will indicate the amount of algae needed, and you could even develop your own protocol. There is no difference between live algae or Easyroti to be used as feed.

A 5 mm diameter rod with very gentle aeration (5-10 bubbles per second) is introduced into the culture recipient to ensure homogeneous mixing of the culture. Excessive turbulence causes rotifer to lose their eggs and they won’t hatch.

It is advisable to distribute the feeding dose in two or more feedings per day, but for time reasons it is usually fed once a day.

DAY 1

Initial conditions: “Strain” culture with 500 ml of sea water.

Number of rotifers: 400 rot./ ml to 600 rot./ ml and 4 ml of Easyroti Nannochloropsis (80 %) + Tetraselmis (20 %).

Final conditions: The culture will have a final volume of 1 L with algae.

Instructions: Disperse 4 ml of Easyroti in 0.5 L of sea water. Add the rotifer inoculum.

DAY 2

Initial conditions: 1 L of rotifer culture. It will look almost transparent and with almost no algae.

Estimated number of rotifers: 500 rot./ ml

Final conditions: The culture will have a final volume of 1.4 L with algae.

Instructions: Disperse 4 ml of Easyroti in 0.5 L of sea water and add 0.4 L to the rotifer culture.

DAY 3

Initial conditions: 1.4 L of rotifer culture. It will look almost transparent and with almost no algae.

Estimated number of rotifers: 500-600 rot./ ml

Final conditions: The culture will have a final volume of 1.8 L with algae.

Instructions: Disperse 8 ml of Easyroti in 0.5 L of sea water and add 0.4 L to the rotifer culture.

DAY 4

Initial conditions: 1.8 L of rotifer culture. It will look almost transparent and with almost no algae.

Estimated number of rotifers: 600 rot./ ml.

Final conditions: The culture will have a final volume of 2 L with algae.

Instructions: Disperse 6 ml of Easyroti in 0.25 L of sea water and add 0.2 L to the rotifer culture.

DAY 5

Initial Conditions: 2 L of rotifer culture. It will look almost transparent and with almost no algae.

Estimated number of rotifers: 700-800 rot./ ml.

From this “Strain” culture, a partial harvest of 0.5 L is done to start a new “Strain” culture in another 2 L recipient and begin a new cycle as on day 1.

The old “Strain” culture, from now on the “Production” culture, we will add Easyroti and bring it up to 2 L. If the container is too dirty, it should be transferred to a clean one to continue the production culture.

Instructions: Add 8 ml of Easyroti previously dispersed in 0.5 L of sea water to the “Production” culture. In the “Strain” culture, start a new cycle from day 1.

DAY 6

Initial conditions: “Strain” culture as on day 2. “Production” culture 2 L with 800 rot./ ml will be almost transparent and with almost no algae.

The “Production” culture will be used to harvest 0.5 L daily to use as food in the aquarium (in case there is no need to feed the aquarium, the harvest should still be done to be able to feed rotifer with algae). Whenever 0.5 L are harvested, 0.5 L have to be added with 8 ml of Easyroti.

The “Production” culture will be maintained until the recipient is dirty (5-7 days).

When the “Strain” culture reaches day 5 it will become a “Production” culture and a new “Strain” culture will be created in parallel, as explained for day 5.

We will empty the old “Production” culture and proceed with the cleaning and disinfection.

Tetraselmis chuii:

  • For its high content of antioxidants Superoxide Dismutase, Catalase and Glutathione Peroxidase.
  • High digestibility for filter feeders and zooplankton.
  • It provides a high content of polysaccharides, polyunsaturated fatty acids (PUFA), pigments and other lipid compounds.
  • Increases the fertility of rotifer.

 

Nannochloropsis gaditana:

  • It provides a lipid profile very rich in EPA and ARA.
  • High content of high quality Omega 3 fatty acids (EPA).
  • Source of vitamins and natural antioxidants.
  • It is the market product, in liquid form, with the highest concentration of cells 5 x 109 cells/ml and also does not require refrigeration during storage to keep all its original nutritional properties intact.
  • Its packaging provides light and oxygen isolation so that the quality of the product is not altered during storage and use.
  • The Easyroti packaging ensures that the shelf life of the product is not affected after it is opened. The flexible bag compresses as the product is extracted, preventing oxygen from entering the container, thus keeping the initial packaging conditions intact.
  • The microalgae that make up Easyroti are cultivated in closed photo-bioreactors under human and animal food safety conditions. These cultivation conditions guarantee the absence of pathogens that could negatively affect a reef ecosystem.
  • Easyroti 250 net content: 250 ml
  • Easyroti prof net content: 1500 ml
  • Easyroti 3000 net content: 3000 ml

For other volumes, please contact the sales department via the following email address: info@easyreefs.com.

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